免疫組織データベース~いむーの



北海道大学病院病理部抗体一覧(2007.4.24 version)

2007年4月24日

Antigbody clone company dilution   magnification retrieval amplification staining   pattern control
34βE12(Cytokeratin HIGH MW) 34βE12 DAKO X100 Pressure cooker × Cytoplasm sausage(prostate)
ACTH polyclonal NICHIREI X3 Pressure cooker × Cytoplasm pituitary gland
Anti chymotrypsin (AACT) polyclonal Zymed X100 Pressure cooker sausage(including pancreas)
Actin, α-Sarcomeric Muscular Actin (Sr-1) Alpha-Sr-1 DAKO X40 Pressure cooker × sausage
Smooth Muscle Actin (SMA) 1A4 DAKO X1000 Pressure cooker × Cytoplasmic sausage
AE1/AE3 (pan-cytokeratin) AE1/AE3 DAKO X70 Enzyme 11min × Cytoplasmic (sometimes with accentuation beneath cell membrane or   paranuclear region sausage
AFP polyclonal DAKO X2000 Pressure cooker × Cytoplasm, granular HCC(AFP positive)
ALK-1 (Anaplastic lymphoma kinase-1) ALK-1 DAKO X50 Pressure cooker Cytoplasmic + nuclear (occasionally pure cytoplasmic) ALKoma
Amyloid A mc1 DAKO X10 Enzyme × amyloidosis
8min
Amyloid P Component polyclonal DAKO X200 Enzyme × Amyloid
(C1t   Complement)
Androgen recepter AR441 DAKO X100 Pressure cooker
Anti-Human Mesothelial cell (HBME-1) HBME-1 DAKO X100 Pressure cooker membrane staining sausage (lung)
αAmylase polyclonal SIGMA X1000 Pressure cooker A/Bあり salivary grand
α- catenin 5 Transduction Laboratories X100 Pressure cooker Cytoplasmic sausage
α-Methylacyl-CoA Racemase 15 Transduction Pressure cooker
Laboratories
α1-AT polyclonal DAKO X10 Pressure cooker × A/Bあり sausage(pancreas)
BAF47 25 Transduction Laboratories X300 Pressure cooker PNET
AT/RT (negative)
Beta-2-Microglobulin polyclonal Novocastra X100 Enzyme × membrane kidney
6min
Beta-Inhibin/Activin 16BA Biogenesis Pressure cooker Granulosa tumors and ovary tissue
Bcl-10 151 Zymed Prediluted Pressure cooker Cytoplasmic + nuclear tonsil
70μl
Bcl-2 oncoprotein 124 DAKO X50 Pressure cooker Perinuclear space sausage
Bcl-6 GI191E/A8 VENTANA Prediluted Pressure cooker Nuclear tonsil
70μl
Blood Group Antigen A 81FR2.2 DAKO X70 Pressure cooker ×
Blood Group   Antigen B 3.00E+07 DAKO X30 Enzyme ×
6min
Blood Group Antigen H 92FRA2 DAKO X70 Pressure cooker ×
Bob-1 Sc-955 SANTA CRUZ X2000 Pressure cooker × Plasmacytoma
BPV-1 (Papillomavirus) polyclonal DAKO X 100~X200
β-catenin 14 Transduction Laboratories X100 Pressure cooker Cytoplasmic sausage
β-PDGFR polyclonal BD biosciences
c-Cbl(p120-cbl) 17 Transduction Laboratories x1000 Pressure cooker tonsil
HER2 CB11 VENTANA Prediluted CC2 Cell membrane breast carcinoma
Cytoplasmic staining should be ignored
c-erbB-2 Poly NICHIREI
c-kit (CD117) polyclonal DAKO X400 Pressure cooker
c-Myc 9.00E+10 SANTA CRUZ
c-myc Oncoprotein 9.00E+11 Novocastra X200 Pressure cooker
CA125 データシート無し CIS bio international X5 Pressure cooker Cell membrane, especially the luminal surface yolk sac tumor
CA19-9 C241 VENTANA Prediluted Pressure cooker ×
Calcitonin CAL-3-F5 DAKO X50 Pressure cooker × Cytoplasm medullary carcinoma (C-cell carcinoma)
Caldesmon, h-CD h-CD DAKO X100 Pressure cooker Cytoplasm and extracellular amyloid
Calretinin polyclonal Zymed Prediluted Pressure cooker Nuclear + Cytoplasmic mesotherial cell
70μl
CALPONIN hCP SIGMA 平滑筋細胞、筋上皮細胞
CAM5.2 CAM5.2 BECTON X5 Pressure cooker CK8+CK18
CD10 Novocastra X40 Pressure cooker tonsil
CD138 MI15 DAKO X50 Pressure cooker Cytoplasmic and membrane tonsil
CD15(Leu-M1) MMA BECTON DICKINSON X5 Pressure cooker tonsil
CD1a O10 DAKO X100 Pressure cooker Golgi with or without cell membrane staining. tonsil
CD21 2G9 Novocastra X50 Pressure cooker × Cell membranne tonsil
CD23 1B12 Novocastra X20 Pressure cooker Cell membrane +/- cytoplasmic. tonsil
CD25 (Human interleukin-2 receptor) 4C9 Novocastra X200 Pressure cooker × Cell membrane +/- cytoplasmic tonsil
CD3 Poly Rabbit DAKO X100 Pressure cooker cytoplasmic tonsil
CD30(Ki-1, Ber-H2) Ber-H2 DAKO X20 Pressure cooker Cytoplasmic, with accentuation in perinuclear space. Sometimes   Golgi staining. Rarely, cell membrane staining. tonsil
CD31(PECAM-1) 1A10 Novocastra X50 Pressure cooker sausage
CD34 NU-4A1 NICHIREI X50 Pressure cooker × Cytoplasm, membrane
CD35 RLB25 Novocastra X40 Pressure cooker × membrane tonsil
CD36(GPIV) SM0 Novocastra X5 Pressure cooker membrane
CD36 63 CASCADE X100
CD4 1F6 Novocastra X15 Pressure cooker membrane tonsil
CD43 MT-1 Novocastra X50 Pressure cooker Cytoplasm, membrane tonsil
CD45 (LCA) 2B11+PD7/26 DAKO X100 Pressure cooke tonsil
CD5 (Leu-1) 4C7 Novocastra X50 Pressure cooker
CD56 (SCLC) 123C3 Zymed Prediluted Pressure cooker Cell membrane tonsil
anti-small   cell lung cancer
CD57(Leu-7) HNK-1 BECTON X15 Pressure cooker × Cell membrane sausage(brain)
CD68(KP-1) KP-1 DAKO X50 Pressure cooker × Cell membrane +/- cytoplasm sausage(brain)
CD68(PGM-1) PGM-1 DAKO X100 Pressure cooker Cytoplasmic, and granular sausage
CD7 CD7-272 Novocastra X50 Pressure cooker tonsil
CD79a JCB117 DAKO X25 Pressure cooker membrane tonsil
CD8 4B11 Novocastra X40 Pressure cooker × Cytoplasmic tonsil
CD9(Motility-Related Protein-1) 72F6 Novocastra X40 Pressure cooker Cell menbrane tonsil
cdk4 DCS-31 BIOSOURCE Pressure cooker
CDX-2 CDX2-88 Biogenex X3 Pressure cooker Nuclear colon
CEA mono ZC23 NICHIREI X20 Pressure cooker × Cell membrane +/- cytoplasmic. Adenocarcinomas
CEA mono COL-1 NICHIREI X20 Pressure cooker × Cell membrane +/- cytoplasmic. Adenocarcinomas
CEA polyclonal KYOWA X100 Pressure cooker × Cell membrane +/- cytoplasmic. Adenocarcinomas
NCA removal
CEA polyclonal DAKO X20 Pressure cooker × Cell membrane +/- cytoplasmic Adenocarcinomas
Chlamydia trachomatis 3 MONOSAN X20 Pressure cooker × Chlamydia trachomatis
Chorionic Gonadotropin (hCG) polyclonal DAKO X400 Pressure cooker placenta
Chromogranin A DAK-A3 DAKO X150 Pressure cooker × cytoplasmic carcinoid tumor
COX-2 Cayman X50 Pressure cooker × Cell membrane
CMV DDG9,CCH2 DAKO X100 Enzyme ×
Cryptococcus neoformans polyclonal DAKO X200 Pressure cooker × Cryptococcus
Cyclooxygenase 2 COX-2 Cayman chemical X50 Pressure cooker Nuclear human breast cancer
Cytokeratin polyclonal NICHIREI Prediluted Enzyme × cytoplasmic
11min
Cytokeratin polyclonal Novocastra X100 Enzyme × cytoplasmic
11min
Cytokeratin 5/6 D5/16 B4 DAKO X200 Pressure cooker × Cytoplasmic sausage
Cytokeratin 7 (CK7) OV-TL12/30 DAKO X50 Pressure cooker × Cytoplasmic sausage(prostate)
Cytokeratin 8 (Cytokeratin Low MW) 35βH11 DAKO X50 Pressure cooker × Cytoplasmic sausage
Cytokeratin 13 KS-1A3 Novocastra X50 Pressure cooker × Cytoplasmic tonsil
Cytokeratin 14 LL002 Novocastra X20 Pressure cooker × Cytoplasmic Skin
Cytokeratin 18 DC10 DAKO X20 Pressure cooker × Cytoplasmic sausage (skin)
Cytokeratin 18 DC-10 Novocastra X40 Pressure cooker Cytoplasmic sausage (skin)
Cytokeratin 19 b170 Novocastra X100 Pressure cooker × Cytoplasmic sausage (skin)
Cytokeratin 20 Ks20.8 DAKO X25 Pressure cooker × Cytoplasmic sausage (skin)
Cyclin D1 SP4 LAB VISION X100 Pressure cooker Nuclear sausage
CXCL13/BLC/BCA-1 53610 R&D Systems X200 Pressure cooker Nuclear tonsil
C4d polyclonal BIOMEDICA X100 Pressure cooker × Cytoplasmic Kidney
D2-40 D2-40 DAKO X50 Pressure cooker × Cytoplasmic and sometimes membrane sausage
Desmin D33 DAKO X100 Pressure cooker Cytoplasmic and sometimes membrane lymphatic endthelium
E-cadherin 4A2 C7 Zymed Prediluted Pressure cooker Cell membrane
EGFR 3C6 VENTANA Prediluted Enzyme Cell membrane and cytoplasm sausage (skin)
8min
Epithelial   Antigen Ber-EP4 DAKO X50 Enzyme Cell membrane sausage (skin)
11min
Epithelial membrane antigen (EMA) E29 DAKO X20 Pressure cooker × Cell membrane +/- weak cytoplasmic adenocarcinoma
Epithelial Related Antigen (MOC-31) MOC-31 DAKO X100 Pressure cooker × Cell membrane and/or cytoplasm sausage
Epstein-Barr Virus, LMP CS.1-4 DAKO X200 Pressure cooker × sausage
EBNA-1 E1-2.5 EXBIO
Epstein-Barr Virus, EBNA2 PE2 DAKO X200 Pressure cooker
ER 6F11 Novocastra X50 Pressure cooker Nuclear breast carcinoma
ER 6F11 VENTANA Prediluted CC1 Nuclear breast carcinoma
Escherichia Coli polyclonal DAKO X1000 -
estragiol polyclonal NICHIREI Prediluted Pressure cooker
Factor VIII related antigen F8/86 DAKO X25 Enzyme
Von   Willebrand factor (VWF) 8min
Fli-1 C19 SANTA CRUZ X150 Pressure cooker × PNET
Follicular Dendritic Cell 〈FDC〉(CNA.42) CNA.42 DAKO X 50 Pressure cooker ×
FSH polyclonal DAKO X5 Pressure cooker × cytoplasmic
gastrin polyclonal NICHIREI Prediluted Pressure cooker × cytoplasmic pituitary gland
GCDFP15(BRST-2) 23A3 Novocastra X100 Pressure cooker ×
G-CSF 4A6 Seikagaku corporation X50 Pressure cooker ×
GFAP polyclonal DAKO X2000 Enzyme ×
8min
Growth Hormone (hGH) polyclonal DAKO X400 Pressure cooker × brain
glucagon polyclonal Novocastra X50 × × Cytoplasmic  A/Bあり pancreas
HCV TORDJI-22 Biogenesis X1000 × Cytoplasmic pancreas
HBV (core antigen) polyclonal Novocastra
HBV(surface antigen) 3.00E+07 DAKO Perinuclear cytoplasm
Hepatocyte OCH1E5 DAKO X100 Pressure cooker Cytoplasmic granules
(HEP-PAR1)
Herpes simplex   virus (type1) HSV-1 20.7.1 Novocastra X25 Enzyme × Nuclear + Cytoplasmic
8min
HHF35(Muscle-specific actin) HHF35 DAKO X100 Pressure cooker ×
HPV polyclonal NICHIREI Prediluted Enzyme × sausage
Anti-phospho-Histone H3 polyclonal upstate X1000 Pressure cooker ×
Human Melanosome HMB45 DAKO X30 Pressure cooker ×
Human Granzyme B GrB-7 MONOSAN X25 Pressure cooker ×
IgG4 HP6025 Zymed X500 Pressure cooker ×
IgA(IHC) VENTANA Prediluted Pressure cooker × Cytoplasmic granules
IgD(IHC) polyclonal DAKO X80 Pressure cooker ×
IgG(IHC) VENTANA Prediluted Pressure cooker  × Cytoplasmic
IgM(IHC) RV69 DAKO X1000 Enzyme ×
Inhibin α R1 DAKO X25 Pressure cooker ×
insulin 2D11-H5 Novocastra X100 × Cytoplasmic  A/Bあり
Kappa Light   Chains A8B5 DAKO X50 Enzyme × Cytoplasmic   A/Bあり tonsil
6min
Ki-67 MIB-1 DAKO X20 Pressure cooker × Nucleus pancreas
L26 (CD20) L26 DAKO X200 Pressure cooker × Cell membrane pancreas
Lambda Light   Chains N10/2 DAKO X400 Enzyme × Cell membrane tonsil
6min
laminin HL-4H3 Fuji chemical X80 Pressure cooker ×
Leukocyte common antigen (LCA) CD45RB (PD7/26) DAKO X200 Pressure cooker tonsil
Lipase 301 Biodesign X2000 Enzyme × Staining pattern: Cell membrane   (pure cytoplasmic staining should not be considered positive) pancreas
LH C93 DAKO X80 Pressure cooker × Cytoplasm
Neurofilament Protein 2F11 DAKO X150 Pressure cooker ×
Neuronal Nuclei(NewN) MAB377 CHEMICON X1000 Pressure cooker × Nucleus
NSE BBS/NC/VI-H14 DAKO X200 Pressure cooker ×
mammaglobin 304-1A5 DAKO X100 Pressure cooker × Breast carcinoma
MDM2 IF2 ZYMED X100 Pressure cooker ×
M-GGMC-1 HIK1083 Kanto chemistry X10 Pressure cooker gastric
Melan-A A103 Novocastra X25 Pressure cooker melanoma
Melanoma NK1/C3 BioGenex Prediluted Pressure cooker × Melanoma
Mesothelin 5B2 Novocastra X20 Pressure cooker membrane staining mesothelioma
Metallothionein E9 Novocastra X25 Pressure cooker × membrane staining Liver or small bowel
Mitochondria MAB1273 CHEMICON X50 Pressure cooker Nuclear + Cytoplasmic
MTP 8 Transduction Laboratories Pressure cooker ×
Muc-1 Core Glycoprotein Ma552 Novocastra X50 Pressure cooker × Cytoplasmic and extracellular membrane-associsted Breast carcinoma
Muc-1 Glycoprotein Ma695 Novocastra X100 Pressure cooker × Cytoplasmic and extracellular membrane-associsted Breast carcinoma
Muc-2 Glycoprotein Ccp58 Novocastra X20 Pressure cooker × Cytoplasmic and extracellular membrane-associated Small bowel
Muc-5AC Glycoprotein CLH2 Novocastra X50 Pressure cooker × Cytoplasmic and extracellular membrane-associated stomach
Muc-6 Glycoprotein CLH5 Novocastra X50 Pressure cooker × Cytoplasmic stomach
MUM-1 MUM1p DAKO X50 Pressure cooker × Cytoplasmic tonsil
Mycobacterium bovis (BCG) polyclonal DAKO X1000 - ×
Myeloperoxidase polyclonal DAKO X10000 Pressure cooker × Cytoplasmic, often granular
Myogenin F5D DAKO X50 Pressure cooker ×
Myoglobin polyclonal DAKO X800 Enzyme × heart
16min
Napsin A TMU-Ad02 IBL X200 Pressure cooker × Cytoplasmic, granular Lung adenocarcinoma
O13(CD99,MIC-2) 1.20E+08 DAKO X50 × × Cytoplasmic Ewing’s sarcoma
2-Oct polyclonal SANTA CRUZ X500 Pressure cooker ×
OCT-3/4 C20 SANTA CRUZ X2000 Pressure cooker × Nuclear goat
Olig2 Affinity Purify polyclonal IBL X100 Pressure cooker × Nuclear oligodendrocyte
p16 protein 6H12 Novocastra X20 Pressure cooker Nuclear
p27 protein 1B4 Novocastra X10 Pressure cooker Nuclear tonsil
PIN Cocktail P504S+p63 BC4A4 (p63) Biocare medical Prediluted Pressure cooker Nuclear (p63) and    cytoplasmic (P504S) prostate carcinoma
P504S 13H4 DAKO X100 Pressure cooker Cytoplasmic prostate carcinoma
p53 protein    (Mutant type) PAb240 DAKO X200 Pressure cooker Nuclear
p53 protein (Wild type) DO-7 Novocastra X300 Pressure cooker Nuclear
p57 57P06 NeoMarkers X200 Pressure cooker Nuclear
p62 polyclonal BIOMOL X1000 Pressure cooker × Cytoplasmic Mallory bodies
p63 4A4 DAKO X100 Pressure cooker Nuclear
Pancreatic polypeptide (PP) polyclonal DAKO X600~X800 Enzyme ×
Pax 5 24 Transduction Laboratories X40 Pressure cooker × tonsil
PCNA PC10 DAKO X200 Pressure cooker × Nuclear tonsil
PDGFR-α polyclonal SANTA CRUZ X300 Pressure cooker × Cytoplasmic tonsil
Perforin δG9 Becton Dickinson X150 Pressure cooker × Granular cytoplasmic tonsil
PgR PgR636 DAKO X150 Pressure cooker Nuclear
PgR 16 VENTANA Prediluted CC1 Nuclear
Placental   Alkaline Phosphatase(PLAP) 8A9 DAKO X400 Pressure cooker × Cytoplasmic
X50 (Brain)
Placental Lactogen (HPL) polyclonal Novocastra X250 Pressure cooker ×
polyomavirus 3.1.1 Novocastra X40 - × Nuclear  and Cytoplasmic BK polyomavirus
prealbumin (Transthyretin) polyclonal DAKO X200 Enzyme ×
PRL polyclonal DAKO X5 Pressure cooker × A/Bあり placenta
Prostatic Acid Phosphatase (PSAP) polyclonal NICHIREI Prediluted Pressure cooker × Cytoplasm prostate
PSA ER-PR8 DAKO X20 Pressure cooker × Cytoplasm prostate
PTEN 6H2.1 CASCADE Bioscience X200 Pressure cooker × Cytoplasm tonsil
PTH 3B3 immundiagnostic X50 Pressure cooker × Membrane pituitary gland
RCC PN-15 Cell Marque Prediluted Pressure cooker × Cytoplasmic, Membrane
S-100 polyclonal DAKO X3000 Pressure cooker × prostate
serotonin polyclonal NICHIREI X5 Pressure cooker × Cytoplasmic + nuclear prostate
Single stranded DNA polyclonal DAKO X400 - × tonsil
somatostatin polyclonal DAKO X8 Enzyme × sausage
Surfactant Apoprotein A PE10 DAKO X100 Pressure cooker × Cytoplasmic
(SP-A)
SV40 T antigen Ab-2 CALBIOCHEM X100 Pressure cooker ×
Synaptophysin 27G12 Novocastra X100 Pressure cooker × Cytoplasmic
TCRα/β-1 (WT31) WT31 Becton Dickinson X20 Pressure cooker ×
TCRγδ(frozen only) IMMUNOTECH X1000 - ×
TdT (Terminal deoxynucleotidyl transferase) polyclonal DAKO X30 Pressure cooker × thymus, ALL
teststeron polyclonal NICHIREI Prediluted Pressure cooker ×
Thrombomodulin 1009 DAKO X100 Enzyme 12min ×
Thyroglobulin DAK-Tg6 DAKO X200 Pressure cooker × Cytoplasm(epitherial cells), membrane(mesotherial cells) thyroid
TIA-1 (cytotoxic granule-associated protein) 26gA10F5 IMMUNOTECH X200 Pressure cooker × Cytoplasm NK cell lymphoma
Tissue Inhibitor of Matrix Metalloproteinase 1   (TIMP-1) 2A5 Novocastra X10 Pressure cooker × Cytoplasmic Macrophages in tonsil
Treponema pallidum polyclonal Biogenesis X1000 Enzyme × TP
TSH polyclonal DAKO X3 Pressure cooker Cytoplasmic thyroid
TTF-1 8G7G3/1 DAKO X200 Pressure cooker × nuclear
Collagen TypeⅣ CIV22 DAKO X100 Pressure cooker + enzyme
UCHL-1 UCHL-1 DAKO X200 Pressure cooker × Cytoplasmic
Vδ1 R9.12 IMMUNOTECH X30 urutra Pressure cooker × tonsil
vimentin V9 DAKO X200 Pressure cooker ×
Wilms’ Tumor 1 (WT-1) 6F-H2 DAKO X20 Pressure cooker × sausage

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